Determination of Doxycycline as Pure and in Pharmaceutical Preparation (Capsule) Using 4-Aminoantipyrine in Presence of Potassium Periodate

A simple spectrophotometric method for the determination of doxycycline in aqueous medium using oxidative coupling reaction has been described. The was based on with 4-aminoantipyrene (4-AAP) which gives soluble and stable red colored product that highest absorption at wavelength 515 nm, linearity Beer's law concentration range from 5 to 110 µg/ml, value coefficient standard curve 0.9995, statistically indicates it excellent linear characteristics. molar absorptivity calculated found be 1.387x103 l.mol-1.cm-1. Sandell's sensitivity value, Limit detection (LOD), ‎‏ limit quantitation (LOQ) were equal 0.3334 μg/cm2, 0.114 0.38 µg/ml respectively successfully applied its pharmaceutical preparation.